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kdgA [2019-07-03 11:53:30]
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kdgA [2019-07-03 11:53:30]

2-dehydro-3-deoxy-phosphogluconate aldolase
locus
BSU22100
pI
5.27
mw
20.72 kDa
protein length
196 aa Sequence Blast
gene length
591 bp Sequence Blast
function
utilization of galacturonic acid
product
2-dehydro-3-deoxy-phosphogluconate aldolase
essential
no
ec
4.1.3.16
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.15|Utilization of hexuronate]

    • Gene

    Coordinates
    2,323,009 2,323,599

    The protein

    Catalyzed reaction/ biological activity

  • 4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate (according to Swiss-Prot)
  • 2-dehydro-3-deoxy-6-phosphate-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate (according to Swiss-Prot)

    • Structure

  • [PDB|1VLW] (from Thermotoga maritima, 41% identity)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|891A32A19D1353BA566FF121BBCA7B986D31D129|kdgR]-[gene|8A0BB2BE0AAAA7FF834A4549E8E2AF343FB10571|kdgK]-[gene|826BFCE6ED2113BB0510E798C6CDB05C4665E0E2|kdgA]-[gene|EB6F40F8651654E0AB09447C5AD670C35A59F261|kdgT]
    description
    [Pubmed|9846747]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9846747], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|891A32A19D1353BA566FF121BBCA7B986D31D129|KdgR]: repression, [Pubmed|9846747], in [regulon|891A32A19D1353BA566FF121BBCA7B986D31D129|KdgR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|9846747], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • induced by galacturonate ([protein|891A32A19D1353BA566FF121BBCA7B986D31D129|KdgR]) [Pubmed|9846747]
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    Biological materials

    Mutant

  • BKE22100 ([gene|826BFCE6ED2113BB0510E798C6CDB05C4665E0E2|kdgA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTTTTCAACGACTTTGGACT, downstream forward: _UP4_TAAGTGGAATTGTTTTTTCA
  • BKK22100 ([gene|826BFCE6ED2113BB0510E798C6CDB05C4665E0E2|kdgA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTTTTCAACGACTTTGGACT, downstream forward: _UP4_TAAGTGGAATTGTTTTTTCA

    • References

  • 9846747